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Hence an isothermal melt at one temperature only was executed for rSBA
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This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure
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We refer to these plots in the following as Guinier plots, due to their analogy to the Guinier plots in SAS
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4 and 5, it can be seen that the Sn-2
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Interestingly, all N-H^sup N^ vectors are also affected by an additional internal motion of ~2
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Mass spectrometryThe BLG preparation was examined by liquid chromatography-mass spectroscopy using reverse phase chromatography with on-line electrospray-ionization mass spectrometry (MicroMass, Cary, NC) as described previously (14)
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Circular dichroismFar ultraviolet circular dichroism (UV CD) spectra were recorded using a Jobin Yvon (Longjumeau, France) circular dichroism spectropolarimeter in the range 190-260 nm
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Protein association to membranes is stabilized by a combination of electrostatics as well as polar and hydrocarbon interactions
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Mature human plasma HDL and LDL are also stabilized by kinetic barriers, suggesting that such barriers provide a universal natural strategy for lipoprotein stabilization (Mehta et al
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Flexural rigidity was measured with an FRL cantilever bending tester (Testing Machines Inc
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The proportions decreased front 0
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5 Spent Ovary flaccid, purplish- Early-stage oocytesred in color
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