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. Unlike simple hyperbolic binding curves, profiles such as those of Fig
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. The linewidth, in turn, is broader in the animal cytoplasm than in the nucleus, but remains constant overtime
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. Incoherent inelastic neutron scattering experiments probing the vibrational density of states are scarce but seem to favor an increase of low-frequency, collective motions resulting in increased protein flexibility upon ligand binding (10)
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. (9), mentioned above, who found that binding of huperzine A to AChE resulted in decreased MSDs
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. Consequently, the on-rate of the equilibrium between free and CaM-bound peptide will be faster than diffusion controlled, which explains the fast exchange and the high affinity observed in the CaM:SEF2-1mp interaction (11)
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. In addition to the homogeneous liquid phases, the conditions under which limited aggregation occurred are also noted
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. In PEG 10000, the concentrations of salts required to collapse DNA were about half those with PEG 3000
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. However, the off-rate of the association was too slow to be physiologically relevant (~2 min), suggesting that other cellular processes might serve to reduce the off-rate
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. Arrhenius plots in(τ) versus 1/T for the two kinetic phases where obtained from the relaxation times τ(T) measured at 70-95°C
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. Thus, the fast unfolding phase is observed in disks and in the mixture of lipid-poor and vesicle-bound apoA-2, whereas the slow unfolding phase is observed in disks only
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