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. Mutational analysis demonstrates that specific electrostatic interactions can play a key role in the denatured ensembles of proteins
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. It is interesting to note that increasing the immersion depth results in decreasing the wetting force and meniscus height
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. At high volume fractions there is a sufficient slowing of particle dynamics such that liquidlike configurations cannot be explored over practical timescales (5,6)
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. At the lower concentrations, Newtonian behavior was observed at shear rates, γ, in the range 10-100 s^sup -1^
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. The Θ^sub 222^(t) data show a rapid signal loss at any Gdm HCl concentration, indicating fast unfolding within the dead-time of the experiment (30 s), which precludes its quantitative analysis; the amplitude of this rapid phase increases with increasing denaturant concentration (Fig
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. Such unspecific effects may contribute to morphological differences in any protein's amyloid samples induced at high temperature
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. This suggestion has recently been made from observations of competing folding pathways in a β -sandwich protein
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