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1 for I7S, and >7
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The study was supported by the European Union program under contracts No
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For this reason, the IMC layer thickness was found from Fig
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Dynamics of protein and peptide hydration
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Furthermore, these data correlate nicely to the crosslinking data of Fig
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4 C shows the data recorded in the first and second jump from 25 to 85°C
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Further support for this hypothesis comes from a large reduction in the apparent T^sub m^ and faster thermal unfolding observed upon disulfide reduction in apoA-2:DMPC disks (data not shown), which is consistent with the report that apoA-2 on the disks is less stable in monomeric than in dimeric form (Lund-Katz et al
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This is of course a restatement of Levinthal's paradox (Levinthal, 1969; Karplus, 1997), but a helpful one, in that the two necessary assumptions for the paradox to arise are clearly seen
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As we have remarked above, however, candidates for such long range forces do not present themselves
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Remarkably, just this conclusion was reached recently by experiments on the helical immunity protein Im7 (Capaldi et al
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A generalized reaction field method for molecular-dynamics simulations
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Acetone and Isopar solvents are miscible, but methanol and Isopar solvents are not
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Hence, quasi-docked and specifically-docked ribozyme species are readily distinguished in our diffusion spFRET experiments
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It was removed intact and without complication
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56) and within each professional year (rfirst-year = -0
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. Interpretations suggesting that highly denatured proteins might he embryos of their native-state counterparts must he treated with extreme caution because there is no sound theoretical basis for such proposals
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. From the changes in the fluorescence intensity, the apparent pK^sub a^ of the titration was 4
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. CONCLUSION It has been demonstrated in this study that the λ^sub max^-values of tryptophan fluorescence emission are meaningful parameter to characterize properties of the unfolded state
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. For example, the CD curve recorded at 1
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