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. The study was supported by the European Union program under contracts No
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. For this reason, the IMC layer thickness was found from Fig
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. Dynamics of protein and peptide hydration
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. Furthermore, these data correlate nicely to the crosslinking data of Fig
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. 4 C shows the data recorded in the first and second jump from 25 to 85°C
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. Further support for this hypothesis comes from a large reduction in the apparent T^sub m^ and faster thermal unfolding observed upon disulfide reduction in apoA-2:DMPC disks (data not shown), which is consistent with the report that apoA-2 on the disks is less stable in monomeric than in dimeric form (Lund-Katz et al
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. This is of course a restatement of Levinthal's paradox (Levinthal, 1969; Karplus, 1997), but a helpful one, in that the two necessary assumptions for the paradox to arise are clearly seen
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. Acetone and Isopar solvents are miscible, but methanol and Isopar solvents are not
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. Hence, quasi-docked and specifically-docked ribozyme species are readily distinguished in our diffusion spFRET experiments
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. Interpretations suggesting that highly denatured proteins might he embryos of their native-state counterparts must he treated with extreme caution because there is no sound theoretical basis for such proposals
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. From the changes in the fluorescence intensity, the apparent pK^sub a^ of the titration was 4
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. CONCLUSION It has been demonstrated in this study that the λ^sub max^-values of tryptophan fluorescence emission are meaningful parameter to characterize properties of the unfolded state
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. Hydration change during the aging of phosphorylated human butyrylcholinesterase: importance of residues aspartate-70 and glutamate-197 in the water network as probed by hydrostatic and osmotic pressures
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.1-10% w/w) with strong shear thinning behavior being observed with increasing shear rate (49-51) and the storage modulus, G', being larger than the loss modulus (49-53), G''
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. As shown in Fig
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. The protein purity assessed by SDS gel electrophoresis was 95%
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. For example, the CD curve recorded at 1
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. Even small (~100 residue) proteins have a configurational space dimensionality of several hundred (think of the bond angles along the polypeptide main chain alone)
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. Is there a unifying mechanism for protein folding? Trends Biochem
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. There is some evidence in the Talmud that rabbis toyed with concepts of probability to determine tax distributions, paternity in cases of adultery, and other Issues where uncertainty played a role
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. HMX-1 also flew the MV-22 in the past, but that unit is no longer involved with the program
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. For instance, Keren and Wagenaar (1987) successfully demonstrated that participants followed the predictions of the utility theory when they played the game repeatedly, but not when they played once
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