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. Infrared spectroscopy The IR samples were prepared by dissolving ACYI in 100 mM deuterated phosphate saline buffer at a concentration of 50 mg/ml
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. Immune enhancement is one area of great potential
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. The protein was expressed and purilied as described (23)
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. pH-induced structural changes Steady-state intrinsic fluorescence measurements
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. To contribute to the ongoing discussion and to investigate the relationship among molecular dynamics, activity, inhibitor-binding, and unfolding, we carried out incoherent elastic neutron scattering experiments on two members of the cholinesterase (ChE) family: Tetrameric human butyrylcholinesterase (HuBChE), both native and covalently inhibited by the organophosphate nerve agent, soman (18), and dimeric native Drosophila melanogaster acetylcholinesterase (DmAChE)
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. Increased protein backbone conformational entropy upon hydrophobic ligand binding
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. Mutation studies on E12 (a bHLH protein similar to SEF2-1) have shown a gradual decrease in CaM affinity upon each mutation of an interacting residue, and not the on-off behavior usually seen with lock-and-key or induced-fit binding mechanisms (15)
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. Another report has demonstrated that Na+ ion nearly doubled the rate of mitosis in cells (Atkinson et al
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. Borén, and U
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. In consequence there are contributions to measured Φ-values that arise from this "preexponential" effect
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. The configurational entropy analysis in terms of combined trajectories presented here shall potentially provide accurate estimations of thermodynamic properties of biomolecules given sufficient sampling of conformational space
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. Each standard concentration was used in triplicate
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. 354350930Medicap Pharmacies, (8) West Des Moines, Iowa348304 95RankCompany, headquarters No
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. The Johns Hopkins team decided to combine the therapies, predicting that the protein would penetrate the liposomes and make them deliver their load of drug more efficiently
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. --Pharmaceutical Sciences Pharmaceutical Health Services Research* MASSACHUSETTSMassachusetts College of Pharmacy and Health Sciences School of Pharmacy-Boston M
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. The ethnicity of the sample was: 61% white, 16
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.Claudia Rosett is a journalist-in-residence with the Foundation for Defense of Democracies
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. The curves from the diluted solution (data not shown) were similar to those presented in Figs
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. Similar CD and turbidity data of apoC-1:DMPC disks were reported earlier (Gursky et al
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