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However, the Fold-X analysis of the modeled EIC^sup sc^ dimer showed differences in the stability and in the interaction energy when compared to those of EIC^sup tt^ (11)
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The excitation light, provided by a Cermax xenon lamp (LX150UV, ILC Technology, Sunnyvale, CA), is selected (λ^sub ex^ = 350 nm for ANS and 285 nm for azurin fluorescence, respectively, 6 nm bandwidth) by a 0
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1 ppm in both compartments
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Therefore, a purely diffusion-controlled on-rate k^sub on^ of 10 s^sup -1^ M^sup -1^ would put a lower limit on the CaM:SEF2-1mp affinity, k^sub off^/k^sub on^ > 10^sup -6^
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71 is plotted in Fig
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5 b) enables our probing the relative hardness of the imaged object
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6) may be a consequence of a more effective relaxation of the mechanical strain (at a similar entropic cost of the assembly)
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Note that there is no "freezing" of other degrees of freedom required whereas those currently active for the search are operating-they are simply making typically ineffective explorations of a much larger subspace
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The process was similar to our process, but a different solvent was used
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At 130°C, the mixtures had to be held for 2 hours for the polymer to appear to be completely dissolved
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020) requiring separate linear regressions to be fitted to the data (Fig
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International Conservation of Bird Preservation Technical Publication 5
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