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The inactivation of E
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Fluorescence measurements were done on a Jobin Yvon Horiha fluorometer (Jobin Yvon Spex, Cedex, France) in a 1-cm cell connected to a circulation water hath (Julabo, Seelbach
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coelicolor, EI^sup sc^, indicate that its thermal denaturation behavior is different from that of other EI proteins, since only one transition is observed (23)
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A test of the model-free formulas: effects of anisotropic rotational diffusion and dimerization
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It is however notable that the ThT molecule also responds to early oligomers (Fig
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013 atm) forces solvent into intersubunit voids resulting in subunit dissociation
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We find that Gα(GDP) significantly decreased the volume change for the CPM-PLCβ-Gβγ association (Table 1)
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. In this research, the sequential events in ACYI thermal denaturation were investigated by a combination of spectroscopic methods and related resolution-enhancing techniques
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. Thermal denaturation of rSBA and gSBA Thermal unfolding profiles of both nonglycosylated and glycosylated SBA were monitored by the change in molar ellipticity value at 218 nm at a protein concentration of 13 µM
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. Here, [left angle bracket]R^sup 2^^sub e^[right angle bracket] is the mean-squared end-to-end distance, R^sup 2^^sub ij^ = |r^sub i^ - r^sub j^|^sup 2^, and we choose r^sub i^ and r^sub j^ to be the position vectors of α-carbon atoms of residues i and j, respectively
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. The interpretation of the RT^sup n^^sub 1^ versus NOE plots is straightforward
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. The observation of 30% helical structure in 5-20 µg/ml apoA-2 in the absence of significant self-association, and the unfolding of this structure upon cooling (Fig
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. Taken together, these data indicate that heating of the apoA-2:DMPC disks to 99°C leads to protein unfolding and disk-to-vesicle fusion; consecutive cooling leads to a gradual reduction in the vesicle size and partial protein refolding, followed by formation of smaller protein-containing vesicles and additional helical refolding at 34-24°C
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. Data fitting suggests that, in contrast to the double-exponential kinetics in the first jump (τ^sub fast^ = 190 s, τ^sub slow^ = 1630 s), the data in the second jump are well approximated by a single exponent with a relaxation time τ = 190 s, which is similar to τ^sub fast^
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. 3, A and B, indicates that, in apoA-2:DMPC complexes, protein unfolding precedes fusion, suggesting only loose coupling
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. This is because the rate-determining diffusive searches will in general be completed only when a topological, as well as a spatial, constraint in the final native state is satisfied for the first time
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. Alternatively, our picture suggests ways of increasing folding times greatly by selective mutations that retain topology and stability of the native state, but destabilize one or more of the on-pathway diffusional subspaces so that intermediate searches are required in d > 3
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.13 kJ K^sup -1^ mol^sup -1^; see Fig
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