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This late phase is concomitant with the spheres shrinking in size, suggesting a conformational change of the spheres into more fibrillar structures
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The environmentally sensitive probe ANS has previously proven useful in detecting molten globule states, starting with the work by Semisotnov and co-workers (Semisotnov et al
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The set of atoms is indicated by the code 2nd
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. These observations suggest that FAD binding is unlikely to play a critical role in the process of GR folding/assembly
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. To look at the generality of the phenomenon, ANS binding experiments were carried out on seven new proteins, namely, monomers (bovine serum albumin (BSA), α-amylase, and β-galactosidase (β-gal)), a dimer (alcohol dehydrogenase from horse liver (LADH)), and tetramers (alcohol dehydrogenase from yeast (YADH), lactic dehydrogenase (LDH), and aldolase (ALD))
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. In either case the conclusion is that in the frozen state the polypeptide structure of the putative binding sites has changed, becoming unable to host ANS or to enhance its fluorescence yield, as when the probe surrounding changes from rigid to mobile (26)
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.Membrane-bound PLCβ appears stable under pressureIn previous work, we found that pressure increased the reaction rate of PLCδ (Rebecchi et al
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.In the present study, we describe development of P
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. Clearly, the four-way helical junction in the native ribozyme is the most important determinant for proper folding, guiding the approach of arms A and B
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