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. Protein concentration used for isothermal melts in lhe fluorimeler was 0
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. The free energy of unfolding calculated for rSBA and gSBA as per LEM model are 41
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. The data show that S [asymptotically =] 0
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. Thermal unfolding of human high-density apolipoprotein A-1: implications for a lipid-tree molten globular state
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. Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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. The fluorescence profiles of all seven proteins in ice, at -13°C, are compared in Fig
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. However, the present data cannot exclude internal motion involving reorientation of the CaM monomers, which have an excepted timescale of ~7-9 ns
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. The approach will additionally help us to see how the existing apparently-distinct paradigms for protein folding are related, and suggest places to look for the information content of the "kinetic code" within proteins that encodes the folding search path, as distinct from the native structure itself
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. This mutation eliminates a tertiary hydrogen bonding interaction between G^sub 11^ (loop A) and A^sub 23^ (loop B) and is expected to destabilize the docked conformer (Rupert and Ferrc-D'Amare, 2001; Klostermeier and Millar, 2002)
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