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Thus, to record adequate emission spectra for GR, we routinely started acquisition of the spectra at 295 nm (as shown in Fig
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ACYI was prepared from pig kidney according to the published procedures (4-6,18)
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The protein concentration used was 0
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8 shows results from our quantitative analysis of cavity statistics for the EV-limit ensembles of proteins
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These observations provide the necessary impetus for developing an accurate statistical thermodynamics framework for understanding how polypeptides respond to increasing concentrations of denaturants to yield ensembles that converge upon the EV limit description
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no histidine residue was near to any of the tyrosine residues or Trp^sup 461^
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The Cu^sub 6^Sn^sub 5^ compounds also appear in the solders other than the solder/Cu interfaces when the concentration of Cu into the molten solder reaches the threshold limit of Cu^sub 6^Sn^sub 5^ formation
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The dominant elasticity results from distortions in the average particle configurations and is thought to be entropic in nature (70)
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For the high-resolution imaging, the AFM head (with optical block and base) was placed atop a commercially available active (piezo-actuated) vibrations-damping desk (from Halcyonics, Göttingen, Germany)
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, 2000), they are the last pre-amyloidal state with a native secondary fold
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. Internal distances between residues that are beyond l^sub s^ should show the same power law dependence on sequence separation as does R^sub g^ (or R^sub e^) on chain length (N)
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. For each of the sequences shown in Table 1
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.When the flat, diffusional degrees of freedom are projected away onto a reaction coordinate pair, such as R^sub g^ and [straight phi]^sub native^, then a folding funnel appears, and does so without the presence of any long-range forces
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.Configurational entropy changes per residueChanges in conformational entropy upon binding can also be calculated per residue
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