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. The underlying assumption is that the highly denatured-state ensemble for two-state proteins can be mimicked using our EV model
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. It is however known that the infective species include assembled prion protein (PrP) oligomers in an aberrant folded state called PrP^sup SC^
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. A Philips CM200 transmission electron microscope operating at 120 kV was used for obtaining micrographs at 100,000-fold × magnification
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. , beyond completion of the second calorimetric peak, leads to large changes in the subsequent scans
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. Under these experimental conditions, no prominent qualitative changes in the elementary morphological folds were observed at times of incubation exceeding 1 h
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. Most of these difficulties are not present if λ^sub max^-signals are employed to characterize unfolding transitions, although these signals can give a skewed assignment of the population of microstates, because a priori the λ^sub max^-signal is not proportional to the population of the states
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