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. ''Today, under very conservative estimates, about 50 percent ofreserves that are held by developing Asia are in excess of what wouldbe required under very adverse conditions,'' Ifzal Ali said, castingdoubt on the current situation in which a major part of thosereserves are held in ''very low-yielding U
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. Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy
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. 2 for the protein ubiquitin
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. Steady-state intrinsic fluorescence Protein samples were excited at 280 and 295 nm between pH 2-12
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. BLG (6 g/L) was dissolved in 0
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. Interfacial rheology of food emulsifiers and proteins
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. These aggregates have a size of ~500 kD, which results in an average concentration of oligomers of ~30 nM
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. , ascribed to it (Bryngelson et al, 1995; Dinner et al
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. Another stripe developed from the dorsal region of the head between the eyes and extended along the dorsal fin, joining the upper lateral stripe at the posterior third part of the body
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. The corresponding conformational entropy differences with their respective standard error estimates from the block averaging analysis are summarized in Table 2
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. For our analyses of fecundity, we used only fish in which the number of eggs or larvae estimated from the three subsamples had coefficients of variation less than or equal to 5%, and for prefertilization eggs we used only females with average egg diameters of at least 450 [mu] to ensure inclusion of all developing oocytes
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. Chondrocytes surplus to clinical requirements for ACI from 24 patients were pelleted and cultured in either DMEM (Dulbecco's modified eagles medium)/ITS+Premix/TGF-[beta]1 or DMEM/10%FCS (fetal calf serum) and were subsequently analysed biochemically and morphologically
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. All participants were required to sign an informed consent statement confirming their willingness to participate in the study that would ask them to report their gambling behaviors, some of which might be illegal in nature
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.0 The scan rates were 30 K/h
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.2 M^sup -1^ and v = 0
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. The use of fluorescence methods to monitor unfolding transitions in proteins
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. On a molar basis, the greatest apparent affinity and the largest values of H are exhibited by the tetramers LDH, YADH, and ALD, the smallest by the monomers azurin, β-gal, and α-amylase
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. This can lower the detection limit of berenil to submillimolar if a whole cell is selected
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. The overexpressed dimeric enzyme has four glycosylation sites per monomer, with carbohydrates accounting for ~10% of the dry weight
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. The glycols or water-soluble polymer materials of WS flux provide close contact between the activator and the substrate surface for heat conduction
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. Being aware that the rotational correlation time of the aggregates were larger than the fluorescence lifetime of bound ANS, we generated a distribution of aggregate sizes using a variety of TTR concentrations from 2 to 8 µM (monomer concentration) that were incubated for 20 h (Fig
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. We find that the K^sub d^ value for this interaction in solution is higher than expected based on a simple model where we calculate protein association on the reduced volume of the membrane
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. Each substrate strand (20 pmole) was incubated with [gamma]^sup 32^ATP (125 pmoles) and T4 polynucleotide kinase (10 U) in 70 mM Tris-HCl (pH 7
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