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. The co-founder of the theory of evolution by natural selection gave an explanation that was, in the end, remarkably Lamarckian
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. Previously we have shown that the native glycoprotein exhibits unusual stability when compared to similar tetrameric legume lectins
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. Since gSBA and rSBA both exist as tetramers with identical ligand binding specificities and macromolecular properties, the change of the mode of unfolding from a two-state process to a three-state process and the decrease of T^sub m^ by 26°C for the thermal unfolding of the protein of the kilter implicate the covalenlly linked glycans for the stability of gSBA
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. There is now increasing evidence that looking at the structure alone is not sufficient to answer these scientific questions (22)
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. Nature 2000; 405:85-90(26) Cikala M, Alexandrova O, David CN, et al
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. Table 1 shows the displacement errors at different rf pulse bandwidths and static magnetic field strengths for the chemical shift span of 5 ppm, the range which covers most of the signals relevant for in vivo ^sup 1^H MRS
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. We note here that although we have interpreted peak shifts and broadening in terms of distance changes and ribozyme subpopulations, respectively, peak shifts could also potentially be caused by solvent effects on dye photophysics, and corresponding changes in the correction factor [gamma] (see Materials and Methods), as well as peak broadening by effects of [kappa]^sup 2^, the orientation factor (Deniz et al
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. The column was then washed extensively with PBS till the washings had A^sub 280^Isothermal guanidinium-hydrochloride-induced denaturation Equilibrium unfolding as a function of guanidinium hydrochloride (GdnCl) concentration was monitored by fluorescence spectroscopy
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. Determination of an ensemble of structures representing the denatured state of the bovine acyl-coenzyme A binding protein
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.The R^sub 1^, R^sub 2^, and NOE data were analyzed using our new analysis method PINATA (22)
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. At this pH the protein has a net negative charge, the dimer carrying a charge of -17e (36), where e is the protonic charge; and so the apparent specific volume would be expected to have a contribution from net repulsive electric double layer interactions in addition to the excluded volume of the hydrated protein itself
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. Using polyethylene glycol solutions as a model of a crowded cell environment, we studied DNA compaction by alkali metal salts LiCl, NaCl, KCl, RbCl, and CsCl, and found that all of these monocations promote DNA compaction
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