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MATERIALS AND METHODSMaterialsGR (type IV, from Baker's yeast), GSSG, NADPH, DTNB, GdnHCl, and HEPES were from Sigma Chemical (St
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General description of dielectric spectroscopy This work was supported by Ministry of Education and Science, grant number 1167203 and CIRTU
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The enzymatic activity was determined by measuring the absorbance at 238 nm accompanied with hydrolysis of the substrate and using the molar absorption coefficient ε^sub 238^ = 185 M^sup -1^cm^sup -1^ as reported by Kordel and Schneider (5,6), except that chloroacetyl-L-leucine was used instead of pure-L-enantiomorph
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Although phase diagrams have usually been used in the analysis of Trp fluorescence data in previous publications, the theoretical analysis of the phase diagram is also expected to be true for IR data
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The structural changes in this stage involved the partial unfolding of the solvent-exposed β-sheet structures and the transformation of about half of the Class I Trp fluorophores to Class II (see Fig
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Again, S = 0 for a perfect sphere
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Folding and association of oligomeric and multimeric proteins
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So our spectra are consistent with the assumption that all of the cytoplasmic diminazene would have been bound to macromolecules and thus give no MR signal
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669 µm for Sn-Ag-Cu-Bi solders) compared to those of the Cu substrates
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Although the other monocations may be involved with DNA binding solely through electrostatic force, there may be some other possibilities with Li+ ion, and the position of Li+ ion in this monocation activity sequence seems to depend strongly on the detection method and experimental conditions
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The detergent-dispersed proteins also showed a K^sub d^(app) ~ 70 nM
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The concentration dependence of binding of these two activators closely matches their respective concentration dependence of enzyme activation (Buck et al
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MATERIALS AND METHODSLipoprotein preparation and characterizationSingle-donor HDLs were isolated from EDTA-treated human plasma by density gradient centrifugation in the range 1
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Afterwards, the second jump from 25 to 85°C was performed, etc
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6 a shows a representative example of the morphological variability in insulin fibrillar aggregates grown for 10 min at 70°C
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Glass transition temperature (T^sub g^) and melting temperature (T^sub m^) were determined by DSC (Seiko SII DSC 200C, Perkin Elmer Pyris I DSC)
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In contrast, the EVA and IPP toners consist of irregular sphere-like particles (Figures 6 and 7)
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Age-related differences in the timing of spawning have been observed in many fishes; usually larger, older fish spawn earlier (Simpson, 1959; Bagenal, 1971; Berkeley and Houde, 1978; Shepherd and Grimes, 1984; Lambert, 1987), but in some cases younger fish spawn earlier in the season (Hutchings and Myers, 1993)
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6 percent less than their male counterparts; an average of 6
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16 an exhibit called "SAFE" that features ClearRx
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Other results showed that 16
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