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The convict weeps not merely because he's in prison, but because he's imprisoned to sin: the sheer meanness of gunning down someone in cold blood just for the hell of it
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. In addition, the peak corresponding to tryptophan emission displayed a significant red-shift to [lambda]^sub max^ ~ 334-339 nm
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. Selective binding and solvent denaturation
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. From these results the following scenario about the underlying processes emerges: Elevated temperatures as well as high concentrations of chemical denaturants dissolve the secondary structure elements and the three-dimensional integrity of the protein structures
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. The results suggest that the stabilization of HuBChE phosphorylated by soman is due to an increase in free energy of the unfolded state due to a decrease in entropy
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. The binding affinity was compared between the different probes defining an intensity 50 value, I^sub 50^, being the half height of the maximum emission intensity, corresponding to an apparent dissociation constant
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. The specific signal obtained from ThT binding to amyloid fibrils should therefore be a combined effect of decreased rotational relaxation and decreased polarity of the surrounding environment
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. When comparing ThT and DCVJ fluorescence data from a protofibrillar sample of TTR (24 h incubation), it is evident that DCVJ shows pHindependent fluorescence (Fig
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. Similar CD and turbidity data of apoC-1:DMPC disks were reported earlier (Gursky et al
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. Such a hierarchical scenario of amyloidogenesis (Aggeli et al
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. These forms vary from slight bends over double turns to semicircles of variable radii of curvature (not smaller than 250 nm)
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. These in turn suggest strategies for protein folding that go beyond those available to native-interactions alone
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. Possible shrinkage was not considered in the measurements
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. Larval abundance was expressed as the number of larvae/ 10 [m
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