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. For instance, we have shown that the thennodynamic folding parameters (ΔC^sub p^ and m-values) of the HPr of S
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. Blank corrections were made in all spectra
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. In solution, this helix is disrupted in the middle (2), which allows N- and C-terminal domains of both apo-CaM and Ca^sup 2+^/CaM to tumble almost independently of each other (3-5)
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. The amount of aggregated material, or the composition of the coexisting phases, was measured by centrifuging the samples for 5 min at 4000 g, removal of the upper phase, and determination of protein content of the aggregates or the concentration in each coexisting phase using spectrophotometry
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. We are grateful to Per Persson for initial assistance with electron microscopy work and to Jeffery Kelly and David Wemmer for providing the pmmHα plasmid
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. Strong experimental evidence is currently emerging that supports all of the main predictions of the approach; other experimental tests of the more surprising conclusions are awaited
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. Legislation passed by Congress in 1992, allows drug makers to pay a fee to speed up the drug approval process
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. Nielsen Media Research found that ESPN's poker viewers largely are men (76
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. " Instead, more than 93 percent of the state's 200-plus communities voted in favor of the lottery
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. The property is a six-story elevator commercial building containing approximately 16,000 s/f
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. When the type of visitors to the gambling web sites were observed itwas found that parents were the most prolific group present
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. Raines set a series of increasingly higher EPS goals that, if met, would trigger bonuses for the executive team that far surpassed what they received in salary
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. Interestingly, considerable conformational changes were observed before the enzyme was fully inactivated by high temperature
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. Towards a complete description of the structural and dynamic properties of the denatured slate of barnase and the role of residual structure in folding
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. The blue edge of the spectra is characterized by larger intensities for the unfolded states as compared to the folded state
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. For example, Fourier transform infrared spectroscopy experiments when conducted on freeze labile proteins have not detected significant changes in their secondary structure (2) presumably because they are carried out at relatively large protein concentrations, a condition in which the perturbation is often attenuated as if the protein itself acted as a stabilizer
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. Bis-ANS shows very strong fluorescence in response to all conformational states of TTR, with the strongest signal emanating from the rapidly formed monomeric A-state upon NaCl addition
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.Characterization of CPM-PLCβ-Gβγ associations at atmospheric pressureIn Fig
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. In 1989 Iowa legislators passed the Excursion Gambling Boat Act, which opened the way for modern riverboat casinos
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.349,210: number of workers employed in commercial casinos in 2004$12
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