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. The IR and fluorescence results strongly suggested that the thermal unfolding of ACYI could be illustrated as a two-stage process involving an intermediate: native state (N) [arrow right] intermediate stale (I) [arrow right] aggregates (A)
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. ΔR^sub ex^ via either set of equations is essentially the same in the fast exchange region
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.Size exclusion chromatography was used to investigate the quaternary structure of GR (Fig
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. Once protein unfolding reached completion in the first jump, the sample was cooled and incubated at 25°C for 1000 s, which was sufficient for completion of the helical refolding (as indicated by flattening of the Θ^sub 222^(t)) but not for the disk reconstitution (as indicated by DSC, EM, and gel electrophoresis)
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