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A reporter's e-mail exchange with Carter Center press secretary Deanna Congileo elicits the response that none of the anonymous donors are from the Middle East, but no further details can be released without permission from the donors--which, even if granted, will take some time to obtain (stay tuned)
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. 5 M GdnHCl and partially folded monomers at 3 M GdnHCl
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. When the sample was heated up to 90°C, immediately cooled, and reheated, the reheating scan had no thermal effect over the temperature range of 30°C-90°C, which suggested that the thermal denaturation of ACYI was fully irreversible
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. Theoretical models for the mechanism of denaturation
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. The three wavelengths yielded the same unfolding curves (data not shown)
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. , limited to the active site), concern low-frequency collective modes below the sensitivity of the technique used and/or present only on a longer timescale (> 1 ns), and not directly related to fast thermal fluctuations
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. In more biochemical language these structures might be exemplified by cooperative secondary structure formation alongside native or near-native distant contacts in α-helix bundle proteins (Myers and Oas, 2001), or simultaneous folding and anion binding (Henkels et al
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. Since a 1 wt% polymer concentration is used, the yield would be low
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. t50%] = length at 50% maturity
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. Our identifying assumption in this and all other regressions is that the pre-RP trends in the outcome variable would have continued into the post-RP period had RP not been implemented
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. Since the MG conformation is more flexible than a fully folded polypeptide, this could lead to better packing at the dimer interface, leading to more stable dimers
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. The content of the Class I component began to decrease when heated to -50°C, and meanwhile the Class II component began to increase and leached a level of ~60% at 66°C
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. Interestingly, the sample with removed GndHCl exhibits now a strong elastic scattering related to pronounced aggregation, which was not observed under unfolding conditions with 8 M GndHCl
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.Detection and Characterization of Aggregates, Prefibrillar Amyloidogenic Oligomers, and Protofibrils Using Fluorescence SpectroscopyLindgren, MikaelABSTRACTTransthyretin (TTR) is a protein linked to a number of different amyloid diseases including senile systemic amyloidosis and familial amyloidotic polyneuropathy
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. For Bis-ANS, the fluorescence in the presence of unfolded TTR is significant
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.t] indicator equals 1 in April 1994 and thereafter (the period during which the Type 1 RP policy was in effect), and equals 0 otherwise; [RP2
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.[ILLUSTRATION OMITTED](L to R) Wal-Mart's Bob Dufour with Natalie Baker and Jim Liebman from AstraZeneca
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