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The phase diagram was constructed by the FSD IR intensity at 1637 cm^sup -1^ versus that at 1628 cm^sup -1^ (Fig
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Previously we have shown that the native glycoprotein exhibits unusual stability when compared to similar tetrameric legume lectins
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For example, scaling of chain size with chain length can provide a direct probe of the nature of chain-solvent interactions (37,38,42,44,45)
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Notwithstanding the relevance of the phenomenon, little is known of the structure of proteins in ice owing primarily to the inapplicability or the poor sensitivity of usual spectroscopic techniques in this highly scattering and anisotropic medium
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Since the dealkylated soman moiety bound to the HuBChE monomer subunit contributes only three hydrogen atoms (P-CH^sub 3^) to the enzyme-inhibitor conjugate, while the deglycosylated native enzyme monomer itself accounts for >3500 nonexchangeable hydrogen atoms, the contribution of the inhibitor is negligibly small
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Ca^sup 2+^ signaling can inhibit the transcriptional activities in vivo of the bHLH proteins E12 and SEF2-1 through direct binding of Ca^sup 2+^/CaM to the basic sequence of the proteins, resulting in inhibition of their DNA binding (15)
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The ^sup 15^N R^sub 1^ and R^sub 2^ experiments at each field were recorded in an interleaved manner with the relaxation delays randomly distributed
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Its characteristic features are: i), specific high-affinity binding in the presence of fast exchange, ii), accommodation of a relatively nonmatching and flexible target via exchange between multiple binding alternatives, iii) dimerization of CaM upon target binding, and iv), large-scale (domain) motions on the nanosecond and microsecond timescale
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The inverse relationship between compaction and binding activities can be explained as follows
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Some extrinsic dyes that by molecular recognition bind to conformational substrates such as native, misfolded monomers and/or aggregates of TTR have been investigated in this work
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6 nm, and the average disk diameter is [left angle bracket]d[right angle bracket] = 11
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Fat crystallization requires about two hours, regardless of the cooling temperature
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However, the herein-observed morphological variation after increasing temperature bears a more profound meaning in light of the ongoing debate as to the identity of the protein's aggregation-prone conformation (completely unfolded, or partly unfolded protein) and our own study, showing that under similar conditions, bovine insulin is substantially more unfolded at 70 than at 60°C (Dzwolak et al, 2003)
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It is, however, not obvious to explain the slight, unexpected gain in entropy upon complexation for gp120 (Fig
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However, based on the observed FRET efficiency, the two loop-carrying arms appear to be relatively far apart in that species, approximately midway between the extended and docked conformers
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(9)Parental attitudes appear to play a significant role in the high incidence of BB and pellet gun possession and use by minors
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com revenues grew from $145 million in 2001 to $246 million in 2003," stated Raman
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