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. Two-dimensional infrared correlation spectroscopy study of the aggregation of cylochrome c in the presence of dimyristoylphosphatidylglycerol
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. Thermal denaturation of rSBA and gSBA Thermal unfolding profiles of both nonglycosylated and glycosylated SBA were monitored by the change in molar ellipticity value at 218 nm at a protein concentration of 13 µM
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. This has been exploited in diffusion-weighted MRS to differentiate between intracellular and extracellular signal contributions of nonwater molecules (17,18)
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. Structural interpretation of the nanosecond-timescale internal motionThe dimeric CaM:SEF2-1mp complex can in principle show internal modes of motion involving either the individual N- and C- terminal domains and/or the CaM monomers as a whole
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. A dramatic difference in the functions of K+ and Na+ has been noted in a large number of biological reports (Strick et al
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. We now take a much simpler fold as an example
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. Clearly this coarse-grained level of model cannot pick up specific and discontinuously large shifts of the transition state, which in small proteins will often dominate particular cases
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. Denaturant-induced movement of the transition state of protein folding revealed by high-pressure stopped-fiow measurements
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. S rangeYearIchthyoplankton surveys1978-79 42[degrees]27'-45[degrees]30'198235[degrees]55'-40[degrees]53'198338[degrees]30'-44[degrees]32'198544[degrees]41'-46[degrees]52'198641[degrees]34'-44[degrees]36'43[degrees]01'-46[degrees]50'199135[degrees]49'-36[degrees]51'199541[degrees]16'-45[degrees]22'199642[degrees]29'-44[degrees]01'199842[degrees]21'-45[degrees]36'199943[degrees]21'-44[degrees]01'200043[degrees]19'-46[degrees]24'200143[degrees]19'-46[degrees]54'42[degrees]54'-45[degrees]25'42[degrees]21'-43[degrees]42'Posttransition juvenile trawls199242[degrees]04'-45[degrees]43'199843[degrees]18'-47[degrees]02'199943[degrees]10'-47[degrees]01'200043[degrees]45'-47[degrees]02'200142[degrees]54'-45[degrees]25'Long
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. 24 kJ K^sup -1^ mol^sup -1^ (Fig
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. Here, we report a method for identifying P
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. But this downgrading of gatekeepers fails to address other issues keeping drugs out of reach
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. ACYI was prepared from pig kidney according to the published procedures (4-6,18)
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. 1 C), with a maximum shifted to lower pH (at pH 6) than that found in fluorescent experiments (Fig
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. The dimerization surface included several salt-bridge networks, such as Asp^sup 467^-Arg^sup 424^', Arg^sup 456^-Asp^sup 358^', Asp^sup 540^-Arg^sup 422^', Asn^sup 345^-Asp^sup 448^', as well as backbone-backbone hydrogen bonds (Fig
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. Principles of Fluorescence Spectroscopy, 2nd ed
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.The backbone structures of the CaM N- and C-terminal domains within the CaM:SEF2-1mp complex are expected to be very similar to those in other CaM structures
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. Interestingly, all N-H^sup N^ vectors are also affected by an additional internal motion of ~2
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. The interaction is clearly different from the well-known categories of specific binding by lock-and-key or induced-fit binding, in which the complex in the bound state becomes essentially locked in one conformation
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. The fluorescence intensity of the different probes in the presence of the unfolded monomer state is shown in Fig
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