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The conformation changes cooperatively from native state to random coil between pH = 5 and 3
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To a biologist, those facts are fascinating--and deeply perplexing
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Theoretical models for the mechanism of denaturation
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6 K), all the measurements are within experimental error, and the differences are probably due to the broadness of the transition
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Although a quantitative relation between aggregation and reduction of the unfolding induced red shifts cannot be given, our data indicate that protein aggregation contributes significantly to smaller magnitudes of red shifts as observed during thermal unfolding
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Following this procedure, reproducible ( Fluorescence measurements Fluorescence measurements were conducted on a homemade fluorometer
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MRI and MRS A homemade MR microscopy probe with a maximum gradient of 10 T/m (23) has been used on a Bruker DMX 600 spectrometer with microimaging system
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Water and lipid MRI of the Xenopus oocyte
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DISCUSSIONIn this study, we have applied high pressure techniques to determine the volume change associated with PLCβ-Gβγ interactions on membrane surfaces and in buffer
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We might note that a pattern of dimensional reduction by nonspecific binding in diffusive searches is a common motif in biology, appearing for example in the search of DNA-binding repressers for their operons (Winter et al
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Large range ofovary dominated by largesize, but all with oocytes with numerousvisible opaque eggs
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1% Tween 20/PBS, and incubated with 4-chloro-1-naphthol solution (substrate of horseradish peroxidase in the kit) containing 0
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These differences were not statistically significant (p > 0
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Bonaventure J, Kadhom N, Cohen-Solal L
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