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. The fluorescence emission of native GR is quite blue-shifted ([lambda]^sub max^ at 317 nm), indicating that, on average, the four tryptophan residues in each GR subunit are well-protected from exposure to the aqueous medium in the folded protein
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. However, quenching experiments and proteolysis patterns suggest conformational changes in the presence of PEP; furthermore, the thermal stability of EI^sup SC^ was modified depending on the effector added
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. the enzyme was more resistant to proteolysis (open and solid circles) (Fig
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. Thus, in contrast to subcellular MRI, we are not aware of any report of subcellular MRS
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. Upon peptide binding, the flexibility of side chains located in the binding sites is redistributed
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. Note, however, that NMR relaxation data on various protein:target complexes do show a redistribution of motions upon specific target binding
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. The normal force had relaxed to RESULTS AND DISCUSSIONPhoton correlation spectroscopyThe conformation and association behavior of BLG are pH dependent (23,24)
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. Being aware that the rotational correlation time of the aggregates were larger than the fluorescence lifetime of bound ANS, we generated a distribution of aggregate sizes using a variety of TTR concentrations from 2 to 8 µM (monomer concentration) that were incubated for 20 h (Fig
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. Similar increase in fluorescence quantum yield was evident in 50% w/w PEG (MW 3350; data not shown) as in 50% glycerol showing that increased viscosity is the main reason for the increased quantum yield
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