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Being aware that the rotational correlation time of the aggregates were larger than the fluorescence lifetime of bound ANS, we generated a distribution of aggregate sizes using a variety of TTR concentrations from 2 to 8 µM (monomer concentration) that were incubated for 20 h (Fig las vegas on line casino gambling .
Similar increase in fluorescence quantum yield was evident in 50% w/w PEG (MW 3350; data not shown) as in 50% glycerol showing that increased viscosity is the main reason for the increased quantum yield las vegas on line casino gambling .
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