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The butanone-rich layer containing heme was discarded
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INTRODUCTION Most proteins are characterized by well-defined three-dimensional structures, which in general exist only within the limits of specific environmental conditions
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Incoherent inelastic neutron scattering experiments probing the vibrational density of states are scarce but seem to favor an increase of low-frequency, collective motions resulting in increased protein flexibility upon ligand binding (10)
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To contribute to the ongoing discussion and to investigate the relationship among molecular dynamics, activity, inhibitor-binding, and unfolding, we carried out incoherent elastic neutron scattering experiments on two members of the cholinesterase (ChE) family: Tetrameric human butyrylcholinesterase (HuBChE), both native and covalently inhibited by the organophosphate nerve agent, soman (18), and dimeric native Drosophila melanogaster acetylcholinesterase (DmAChE)
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A control comparison of HuBChE-H2O and HuBChE-D^sub 2^O (not shown) revealed identical MSDs below 260 K, but higher MSDs of the H2O-hydrated sample at higher temperatures
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For this reason, the IMC layer thickness was found from Fig
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As described in the Theory section, k^sub ex^ can be derived from the correlation between R^sub ex^ and ΔR^sub ex^ without prior assumption on the timescale of the exchange
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48), and the anus was situated near the midpoint of the body (Fig
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Holmberg; OB = RV Capitan OcaBalda; SM = RV Shinkai Maru
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The intermolecular interaction involves stable hydrophobic contacts with a unique protruding CD4-Phe43 structure surrounded by an intermolecular hydrogen-bond network that covers the hemisphere of the CD4 D1 domain
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, unequal slopes) between stage-specific absolute fecundity and age (2-tailed t-test, P=0
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3 years) completed the Gambling Motivation Scale (GMS) and a measure to investigate the level of gambling involvement
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