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The unite probability of linding large cavities within the interior of denatured proteins emphasizes two points: First, the volume occupied by a chain is filled inefficiently when compared to either a folded protein or a freely rotating chain
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We attempted, by docking of the EIN^sup sc^ and EIC^sup sc^ domains, to model the whole structure of EI^sup sc^ trying to keep the active-site histidine (in the EIN^sup sc^ domain) close to the PEP binding site (in the EIC^sup sc^ domain)
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25 m grating monochromator (H-25, Jobin-Yvon, Longjumeau, France) set to a bandwidth of 3 nm
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The relaxation data were measured at multiple magnetic fields, and analyzed in a model-free manner using in-house written software designed to detect nanosecond internal motion
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The experiments were carried out on Bruker DRX-400, AMX2-500, and DRX-600 spectrometers equipped with triple-resonance (^sup 1^H/^sup 13^C/^sup 15^N or broadband) probes with XYZ-gradient capabilities
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As expected from Fig
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The second virial coefficient is negative in this pH region, indicative of there being short-ranged attractive forces
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Native tetrameric TTR can serve as a reference state to which some, but not all, fluorescence probes used in this study can bind
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A surprising result is the rank order of the kinetic disk stability ΔG* ~ln τ that is evident from the timescales of the heat denaturation, apoA-1 > apoA-2 > apoC-1 (Fig
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The disadvantage of the projection is that the subtle origin of the directed search is obscured
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All specimens examined had 33-40 total myomeres (mode:36-38 myomeres)
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The influence of cooling conditions on particle size and shape is summarized in Table V
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major eggs in seawater
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Because faculty members most often observe students during class time, the results of this observation could imply that students experience their largest amount of stress during class hours (Misra, et al
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