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. Each of the subunits is glycosylated at the Asn-75 position (Fig
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. Hence, for any reasonable value of Δ^sup ex^ (up to 6 ppm), these residues are in fast exchange (k^sup -1^^sub ex^ [much greater than] Δω^sub I^)
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. Its characteristic features are: i), specific high-affinity binding in the presence of fast exchange, ii), accommodation of a relatively nonmatching and flexible target via exchange between multiple binding alternatives, iii) dimerization of CaM upon target binding, and iv), large-scale (domain) motions on the nanosecond and microsecond timescale
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. The DSC curves were obtained using a scanning rate of 0
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. Furthermore, studies with solubilizing agents such as detergents, reversed micelles, or nondetergent sulfobetaines (NDSB) proved that aggregation, which typically occurs during thermal unfolding in pure buffers, is strongly suppressed when such agents are added to the solution (29,32)
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. The individual response to freezing differs considerably, on the other hand, with regard to the number of binding sites n increasing two and three times relative to C3A/C26A in I7S and F110S, respectively
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