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. Intrinsic fluorescence spectroscopy The samples for the fluorescence measurements were prepared by dissolved ACYI in 30 mM Tris-HCl (pH 7
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. Each of the subunits is glycosylated at the Asn-75 position (Fig
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. The favorable interactions between the protein and the glycans thus obtained were grouped in two categories, hydrogenbonding and hydrophobic
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. Characterization of a urea induced molten globule intermediate state of glutaminyl-tRNA synthetase from Escherichia coli
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. 5 ms and two 180° pulses of 0
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. For prolate ellipsoids of this aspect ratio, the maximum packing fraction is ~0
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. 5M ammonium hydroxide (9:7:2, v/v) under nonreducing conditions
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- To help minimize surprises, assume no cash issue is too basic
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. The attempts to avoid artifacts in the 2D IR plots have been described in detail elsewhere (30)
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. The slit widths were 3 and 5 nm for emission and excitation, respectively
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. The number and position of bands obtained from the deconvoluted (with a Lorentzian bandwidth of 18 cm^sup -1^ and a resolution enhancement factor of 2) and the Fourier derivative (with a power of 3 and a breakpoint of 0
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.The variation in (τ^sup o^^sub m^)^sub app^ in Fig
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. This is based on the observations that the bound SEF2-lmp lacks any well-defined conformation, and that not all hydrophobic peptide residues simultaneously interact with the hydrophobic patches on the inner surface of the CaM dimer (11)
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. Redistribution and loss of side chain entropy upon formation of a calmodulin-peptide complex
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. The rate of probable pathological gambling was higher than that found at other campuses, and slightly higher than that in previous reports of UNLV underage students (Oster & Knapp, 2001)
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