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. Runs were carried out at 23°C on a Shimadzu (Kyoto, Japan) LC-10AS HPLC at a flow rate of 0
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. An additional experiment in which the incubation time of the protein in the presence of 3 M GdnHCl was increased from 90 min to 24 h showed that the small dimer fraction observed in Fig
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. As the temperature was increased, the intensity of the hands from the native secondary structures decreased, and two new bands (1618 and 1682 cm^sup -1^) appeared at temperatures above 70°C
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. The sequence of events can be analyzed by comparing the signs of the corresponding crosspeaks in the synchronous and asynchronous plots according to the rules proposed by Noda (29)
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. Low temperature induced conformational changes of aminoacylase
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. All sequence-specificity is localized to the high-q, short-wavelength regime
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. This property is only related to the chemical nature of the flux and additives and is independent of the solid contents of the flux
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. 5) or 40 mM Na-acetate (pH 3
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. 5 min): generation of the A-state monomer; 2), first aggregation phase (0
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. In contrast, repetitive DSC scans recorded from the same sample are significantly different
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. The dorsal and anal fin elements reached their full complement by 9-10 mm BL, whereas the caudal-, pelvic-, and pectoral-fin elements were still incomplete in the size range analyzed in this study (3
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. 99 w/d38Table 5Positive stations for posttransition pinguipedids in the Argentine Sea,during 1992-2001
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