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. Furthermore, fluorescence spectral shift data suggested that the unfolding of GR was complete at 3 M GdnHCl (Fig
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.2] at room temperature for 15 minutes
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. This result confirms that the sizable increases in E with Mg^sup 2+^ that we observe for the 2WJ (WT and SL) and SL 4WJ ribozymes are due to decreases in the interarm distances
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