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"Aboard Isabel S
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. Apart from its crucial functions in intracellular amino acid metabolism, ACYI also has substantial commercial importance for the optical resolution of N-acylated DL-amino acids
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. Cold denaturation-induced conformational changes in phosphoglycerate kinase from yeast
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. 5 to 4 µM, and only KI was used as the quencher
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. Recently, a study conducted in our laboratory investigated the possibility to unveil potential alterations of the protein tertiary structure in ice from binding of ANS (12)
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. NaCl suprapur was purchased from Merck (Darmstadt, Germany) and ANS from Sigma (St
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. Possibly the dimeric nature of both the target and CaM overcomes the less specific hydrophobic interactions
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. The interaction neither belongs to the category of lock-and-key nor to that of induced-fit
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. In the analysis of the CaM data we used an axially symmetric diffusion model to calculate the relaxation parameters
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. 4-angstrom structure of calmodulin-peptide complex
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. We find that the association of Gβ86-105 to PLCβ gives a volume change that is similar to the full Gβγ-subunit
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. The gp120 molecule without the V3 loop and the CD4 molecule, both using all atoms (code: all) or using only backbone (N, Cα, and C') atoms (code: bb); see Fig
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. Also, of the study's 152 participants, 91
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