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Secondary structure of proteins from circular dichroism spectra
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Detection and characterization of the intermediate on the folding pathway of human alpha-lactalbumin
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The gene mapping showed that at least three genes, on three distinct chromosomes, affect eye development, though the exact roles of the genes are still unknown
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The asynchronous plot was different from that in Fig
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Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy
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Although there were changes in the tertiary structure, EI^sup sc^ retained some, if not all, of its secondary structure, as shown by the increase (in absolute value) of the [Θ] at 222 nm (Fig
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Accordingly, the minimum number of bound ANS molecules (n) increases from 1
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Looking at the first portion of the righthand side of Eq
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Most HLH proteins belong to the bHLH group, which has a basic sequence directly N-terminal to the HLH motif
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We find that residues with significant exchange broadening (Table 1) group in a model structure of the CaM:SEF2-1mp complex into three regions: i), the CaM dimerization interface, i
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For small applied stresses, the material has the solidlike characteristics of a glass with the particles forming jammed structures that are stress bearing
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Thus, although the general mechanism of kinetic stabilization is similar for HDL disks and spheres, the molecular determinants for their stability are significantly different
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8 59OB-05/0065[degrees]01'Pseudopercis1388
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After immunostaining the eggs were observed by stereoscopic microscopy (SMZ-2T, Nikon Co
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The sale of Albertsons
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Next came scratch-off tickets, advertising on TV and radio, multi-state lotteries with bigger jackpots
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. The protein was expressed and purilied as described (23)
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. Fast and one-step folding of closely and distant related homologous proteins of a four-helix bundle family
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. Sample preparation Before sample preparation, all protein stocks were dialyzed against 2 mM Hepes buffer, pH 7
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.) that are likely to require completely different dynamical behavior on an extended timescale
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.-Department of Electronic Engineering, City University of Hong Kong, Kowloon, Hong Kong
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.6 [Angstrom] when the backbones from N- and C-terminal domains from eight other CaM molecules were compared (data not shown)
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. Nevertheless, the trends remain correct, but the ΔH^sup #^ numbers should be considered with proper care
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. Clearly this coarse-grained level of model cannot pick up specific and discontinuously large shifts of the transition state, which in small proteins will often dominate particular cases
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. Frenkel for helpful discussions and comments on the manuscript, and the Kavli Institute for Theoretical Physics for the hospitality of its program "Dynamics of Complex and Macromolecular Fluids", where the bulk of this work was done (preprint number NSF-ITP-02-55)
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. By contrast, abundant matrix and collagen fibrils were observed in the pericellular region of pellets cultured in DMEM/ITS+/TGF-[beta]1 (Figs 5b and c)
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. The interaction was again not significant, F (1,140) = 2
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