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. GR is a 108-kDa, homodimeric flavoenzyme that catalyzes the [beta]-nicotinamide-adenine dinucleotide phosphate (NADPH)-dependent reduction of oxidized glutathione (GSSG) to its reduced form (GSH), thus participating in cellular defense mechanisms against oxidative stress
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. Table I lists relevant information for the 23 protein sequences (68) used in this study
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. and thus the molten-globule of EI^sup sc^ could have some of its dimeric interface partially disrupted
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. All α-amylase structures bear multiple tryptophan residues more or less equally distributed over the whole structure (see Table 1 and Fig
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. Optically dense samples were corrected for inner filter attenuation of the intensity, which was typicallyRESULTS AND DISCUSSION Ice-induced enhancement of ANS fluorescence by WT and mutant apo-azurins Azurin is a 14 kDa copper-binding protein with an eight-stranded β-sandwich structure about a highly hydrophobic core (25)
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. Equation 2 implies that when contact angle θ^sub c^ increases, the surface tension force moves toward negatives due to the cosine value of θ^sub c^, resulting in the negative wetting force
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.Protein-lipid complexes were visualized by negative staining EM in a CM12 transmission electron microscope (Philips Electron Optics, Eindhoven, the Netherlands) as described (Gursky et al
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.Similar to the CD data, the light-scattering heating curves of the apoA-2:DMPC disks shift to lower temperatures at slower scan rates, indicating high activation energy for the disk fusion
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. The FRET efficiency histograms described in this article were generated by using a two-channel data collection mode to simultaneously record donor and acceptor signals as a function of time, with a time resolution of 0
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. Extended and docked conformations of the hairpin ribozyme were also resolved and quantified in previous ensemble trFRET experiments (N
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