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Based on mass spectromctric results, a structural model of ACYI was proposed, and it was suggested that each ACYI subunit might be composed of two domains with α/β folds: the catalytic domain and the dimerizalion domain (17)
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J Clin Invest 2002; 109:661-670(9) Hansen PR, Holm AM, Svendsen UG, et al
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The phosphatidylserine receptor from Hydra is a nuclear protein with potential Fe(II) dependent oxygenase activity
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The fact that for NDSB samples the obtained Δλ^sub max^-value between the folded and the unfolded state is much larger as compared to that measured with pure buffer samples is a further indication that aggregates reduce the magnitude of red shifts
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In a second experiment, we compared dimeric DmAChE and tetrameric HuBChE
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Circular dichroism experiments were carried out on the DmAChE sample before and after the neutron scattering experiment, to see if any conformational change had occurred
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Unlike PH-PLCδ^sub 1^, the membrane binding face and orientation of PH-PLCβ^sub 2^ are unknown
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Regulation of the rate and extent of phospholipase C-β^sub 2^ effector activation of the βγ subunits of heterotrimeric G-proteins
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These in turn suggest strategies for protein folding that go beyond those available to native-interactions alone
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Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody
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5 shows selected histograms from these measurements from 0
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. "Squeezing it gives you a cooling-off period, which lessens your chances of making an unplanned purchase," says Karen Gross, J
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. Third, a slightly brown colored aqueous layer was dialyzed against water until the pH of the protein solution became 5
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. On freezing of the solution, however, the protein sharply enhances the probe fluorescence and shifts its spectrum to the blue
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. So our spectra are consistent with the assumption that all of the cytoplasmic diminazene would have been bound to macromolecules and thus give no MR signal
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. The experiments were carried out on Bruker DRX-400, AMX2-500, and DRX-600 spectrometers equipped with triple-resonance (^sup 1^H/^sup 13^C/^sup 15^N or broadband) probes with XYZ-gradient capabilities
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. The exchange data for all residues are summarized in Fig
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. In the 70°C samples, semicircles and sinusoidal bends are observed
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.Glass transition temperature (T^sub g^) and melting temperature (T^sub m^) were determined by DSC (Seiko SII DSC 200C, Perkin Elmer Pyris I DSC)
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. No color was apparent in the solvent when it was removed from the gel after TIPS
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. Length is a better predictor of fecundity than age as judged by the goodness-of-fit of the multiple linear regression model (Fig
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