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. As apparent from dynamic light scattering studies, the intermediate observed during unfolding of rSBA is monomeric in nature
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. This increase in the helicity was further confirmed by the FTIR experiments carried out at pH 10 (Table 2)
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. 9 µeV, corresponding to a time window of ~730 ps; accessible Q-range: 0
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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. Arrhenius plots for these phases are linear and intercept near 100°C (Fig
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