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MATERIALS AND METHODS Materials Imidazole, the different salts and acids used in buffer preparation, sodium pyruvate, and NaCl were from Sigma (St
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However, the Fold-X analysis of the modeled EIC^sup sc^ dimer showed differences in the stability and in the interaction energy when compared to those of EIC^sup tt^ (11)
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1), the calculated R^sub h^ of both the protein in solution and its aggregate increased in size and was correlated to the phase separation/precipitation of BLG from aqueous solution at these concentrations and ionic strength (34)
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The PH domains of the enzymes are responsible for their different membrane binding characteristics; PLCδ^sub 1^ and its isolated PH domain binds specifically to membranes containing its substrate PI(4,5)P^sub 2^ through a series of specific hydrogen and ionic bonds (Ferguson et al
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, the ingredient subcords are of equal length and are evenly aligned
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Ribozyme constructs missing the substrate strand were prepared in annealing buffer (missing NaCl and supplemented with 12 mM MgCl^sub 2^) at a concentration of 2 µM of each strand and annealed by heating to 70°C for 2 min and cooling to ambient temperature
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041Effect of Type 2 RPCumulative effect over all months $4,637,901<
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