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. (11) used small-angle x-ray scattering (SAXS) to measure R^sub g^ as a function of N for 28 different chemically denatured proteins, with chain lengths varying from 8 to 549 residues
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. The wave numbers used in the calculations range from q = 0 to q = 0
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. J Immunol 2006; (in press)(57) Lee JH, Lee DS, Kim EK, et al
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. On repeating the process we found a consistent decrease in fluorescence intensity at 300 nm accompanied by an emerging red component in the spectrum and enhanced scattering from the solution, the latter response revealing partial unfolding of the macromolecule with exposure of buried W48 to the solvent and concomitant formation of protein aggregates
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. Calculations (21) show that if a longer ranged attraction is added on to the AHS model, although the percolation line does not move, the effective stickiness varies such that the liquid-liquid coexistence line is no longer below the percolation line, a potential explanation for the absence of particle gelation
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. To enable characterization of different conformational states of TTR, we employed four different molecular probes: ANS, 4-4-bis-1-phenylamino-8-naphthalene sulfonate (Bis-ANS), 4-(dicyanovinyl)-julolidine (DCVJ), and thioflavin T (ThT) (Fig
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. Similar to the results obtained for the proteins labeled with other probes, the titration curves for the proteins labeled with CPM showed the appropriate shift in midpoint when the initial concentration of CPM-labeled protein was changed, thus showing that the changes in coumarin fluorescence reflect protein-protein associations
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. Thus, they would be expected to compress with the hydrocarbon chains of the membrane interior and be stabilized under pressure
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. If this interpretation is correct, then the data in Fig
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. © 2005 by the Biophysical Society0006-3495/05/02/1344/10 $2
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. The corresponding conformational entropy differences with their respective standard error estimates from the block averaging analysis are summarized in Table 2
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. Samples containing 4 x 10^sup 5^ cells were centrifuged at 500 g in 15 ml polypropylene conical tubes
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