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. Hence, we can conclude that the intermediate obtained in the chaotrope-induced denaturation of rSBA is also monomeric in nature
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. NMR microscopy of single neurons using spin-echo and line narrowed 2DFT imaging
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. 5 in the presence of 80% glycerol clearly shows pH-independent fluorescence (Fig
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. DCVJ was found to be very sensitive to formation of early oligomers and showed the strongest apparent binding affinity of the probes in the study
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. Therefore, throughout the following overnight dry incubation period no further structural transitions are likely to occur in the amyloid samples
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. The AFM image of the fibroid of the total length of 544 ± 12 nm may be interpreted as either a periodically twisted structure with a regular helical thread of 52 ± 2 nm, or linearly-staggered elements, each 52 ± 2 nm long, constituting a longer topological block
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. Furthermore, it appears when the interactions generating coil-collapse are projected along an ordinate of sequential subspaces, rather than along a spatial coordinate
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. Resin blocks were further trimmed after which 90 nm sections for TEM were cut with a LKB Ultratome III ultramicrotome using a diamond knife
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. INTRODUCTION Aminoacylase, a family of metalloenzymes found in many mammalian tissues and in microorganisms, catalyzes the stereospecific hydrolysis of acylamino acids to yield the corresponding organic acid and amino acid (1-3)
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. Our data therefore indicate that unfolded states exhibit a larger red shift of the fluorescence emission spectrum when theses states under the respective conditions are less susceptible for aggregation
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., in photosynthesis) to folding processes that can take minutes or longer (1)
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. In the coexistence region (Fig
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