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. The PK^sub a^, leading to the acidic species could be determined from the ANSbinding and intrinsic fluorescence measurements, suggesting that one aspartic and/or glutamic acid must be responsible for this transition (43)
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. For PPA, which already exposes tryptophan residues to the protein surface in the folded state, a blue shift of the emission spectrum occurs upon thermal unfolding (see Fig
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. Most interestingly, they found that a substantial part of the AChE molecule is involved in the gorge opening and not just a few gorge residues
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. 93 [Angstrom]^sup -1^) at the Institute Laue Langevin
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. The tetrameric band at 55 kD originates from the amount of unfolded monomers that have not yet been misfolded/misassembled and can refold instead of forming aggregates during the pH jump (from pH 2 to pH 7
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