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. The results are shown in Fig
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. In contrast, RhoA induces the formation of stress fibers, focal adhesions, and cell spreading
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. Due to static and dynamic quenching of solvent molecules or nearby intramolecular subgroups, the intensity of the fluorescently emitted light can vary significantly
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. Upon freezing the sample, the ANS intensity first decreases in the low concentration range, below 80 µM, but then rises sharply above this threshold
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. The aggregation process produced fairly monodisperse oligomers (300-500 kD) within 2 h that matured after 20 h into larger spherical clusters (30-50 nm in diameter) and protofibrils as shown by transmission electron microscopy
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. A schematic representation of the TTR misfolding reaction from the unfolded state through the A-state is presented in Fig
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. The environmentally sensitive probe ANS has previously proven useful in detecting molten globule states, starting with the work by Semisotnov and co-workers (Semisotnov et al
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. The majority of mature amyloids are unbranched fibrils (Rochet and Lansbury, 2000) consisting of several, often intertwined protofilaments (which may form an internal waterfilled canal-Perutz et al
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. A complementary approach is to characterize the conformational distributions of freely diffusing RNA molecules using a single-molecule method referred to as diffusion spFRET (Deniz et al
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.5 kbar (data not shown), a pressure at which a large number of protein dimers and higher oligomers have been found to undergo subunit dissociation (Gross and Jaenicke, 1994; Heremans and Smeller, 1998)
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. Further observations on the electrical properties of hemoglobin-bound water
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.009 × R × (M - 1) ([(R - n) × 0
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.Figure 5c reveals that WS flux results in better wettability among the three fluxes on the Ni substrate
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. This motion may or may not be superimposed onto varying degrees of picosecond-timescale motion
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.5 there is significant protein dimerization
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.0) will dissociate TTR into unfolded monomers (Lai et al
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. This lack of pressure stabilization may be due to the inability of the PH-PLCδ^sub 1^ to further penetrate the membrane surface to allow for stronger hydrogen bonding and ionic interactions
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