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Since no high-resolution structure of ACYI is available, all of the Trp fluorophores (Classes A and S, I, II, and III) defined previously (34) were included in the fitting procedure
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In this model, the polymer is treated as a cloud of uncorrelated monomers in a mean field
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Cell Death Differ 2004; 11:1258-1264(41) Morimoto K, Amano H, Sonoda F, et al
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Although for BSA a high binding capacity may be expected from its intrinsic tendency to bind nonpolar and aromatic moieties also in the liquid state, the above ANS binding data do suggest that beyond specific trends of individual polypeptides, multi-subunit proteins are more susceptible than monomers to destabilization and partial unfolding in the frozen state
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Although subcellular variations could not be determined with this whole-cell technique, it yielded spectra with increased resolution and sensitivity, since dipolar broadening and susceptibility variation within the sample are being reduced (30)
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Interestingly, we found that ANS fluorescence intensity increases as the A-state molten globule of TTR aggregates
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The resulting Θ^sub 222^(t) data, which showed CD changes during the dead-time of the experiment (Differential scanning calorimetryAn upgraded MC-2 microcalorimeter (Microcal, Amherst, MA) was used to record excess heat capacity of the apoA-2:DMPC complexes
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A total of 11 to 18 melanophores, about one melanophore per anal fin pterygiophore, was observed in postflexion larvae (Fig
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They were dehydrated sequentially in a series of graded alcohol washes, cleared in xylene prior to embedding in paraffin
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