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in economics from Rollins College
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The values of K^sub SV^ for Trp residues in proteins and for free Trp in aqueous solution are not directly comparable
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This conclusion has been reached through quantitative studies of the scaling of hydrodynamic radii (46) and radii of gyration (11,47) with chain length under harshly denaturing conditions
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Then, the experiments monitoring protein stability under different conditions were carried out at protein concentrations ≥4 µM
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If, for example, a tryptophan residue becomes more accessible to the solvent, which quite often is the case for unfolded states, the emission intensity decreases
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Copper-free azurins (apoazurins) were prepared from holo-azurins by adding potassium cyanide and EDTA to final concentrations of 0
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© 2006 by the Biophysical Society 0006-3495/06/03/1797/07 $2
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Since the dealkylated soman moiety bound to the HuBChE monomer subunit contributes only three hydrogen atoms (P-CH^sub 3^) to the enzyme-inhibitor conjugate, while the deglycosylated native enzyme monomer itself accounts for >3500 nonexchangeable hydrogen atoms, the contribution of the inhibitor is negligibly small
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Dynamics on micro- to millisecond timescaleThree different approaches were used to determine which CaM residues in the CaM:SEF2-1mp complex are affected by conformational exchange
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Thus, the dynamic features presented here support the conclusion that the conformationally heterogeneous SEF2-1mp, trapped inside the CaM dimer, constantly exchanges between different binding sites
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1 in the concentration range 45-54% w/w was determined by oscillatory rheometry
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As with the PEG 3000 sample in Fig
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A Philips CM200 transmission electron microscope operating at 120 kV was used for obtaining micrographs at 100,000-fold × magnification
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The membrane binding behavior of Gβγ and Gα(GDP) contrasted with that of the PLCβ
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As we determined earlier, this preliminary stage of the aggregation process is endothermic (Dzwolak et al
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By combining the trajectories of these states, however, more information can be obtained
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The observation of well-resolved subpopulations in the FRET efficiency histogram indicates that the ribozyme can adopt two distinct conformations in solution
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The amounts of both breakage and dissolution are greater, for example, in juvenile owls (Andrews 1990), resulting in greater bone loss than in pellets from adult owls (Raczynski and Ruprecht 1974)
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And as if this list isn't long enough for you, I have six more macro retail-specific trends for 2006 that will impact retail pharmacy and continue to change the competitive landscape of this viable industry
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"In the states where pharmacists can provide emergency contraceptive advice--Washington and California--we do charge $15," he said
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. The fitting of the fluorescence spectra was performed using the discrete states model of Trp residues in proteins and was calculated by a program developed in-house (30) based on the SIMS algorithms of decomposition (32)
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.Fast exchange on the NMR timescale indicates off rates k^sub off^ > 2 Δδ^sub N^, which for the CaM:SEF2-1mp complex is ~30 s^sup -1^ (Table 1)
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. Direct observation of the coil-globule transition in DNA molecules
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. In the extreme, protein instability produces serum separation in the package and/or a meltdown that is curdy and shows whey separation
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. Such unspecific effects may contribute to morphological differences in any protein's amyloid samples induced at high temperature
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