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Bob Ney (R-Ohio) pleaded guilty in September to selling legislative favors to clients of the disgraced lobbyist Jack Abramoff, it signaled that the fallout from the corruption scandal is far from over
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. 1 A) and the maximum wavelength of the emission spectra moved toward higher values (data not shown), indicating solvent exposure of the fluorescence residues
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. 5 c, and as known for all α-amylases studied here, the protein concentration has no effect on the transition temperatures (17)
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. For the drug uptake experiments, the oocyte was then immersed in an aqueous solution of 250 mM diminaze aceturate (Sigma) and inserted into the MR tube loaded with the same solution
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. This ~90° rotation of the diffusion tensor together with the relatively small structural changes within each CaM domain puts direct restraints on the overall structure of the CaM:SEF2-1mp complex, which allows us to choose one of the two possible schematic structure models proposed in our earlier study (11) (see Figs
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. At higher pHs there is the potential for a time-dependent irreversible aggregation
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. 0 no salt), and soluble misfolded oligomeric state (pH 2
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. 5 ns pulses, for time-resolved measurements of fluorescence decay and anisotropy
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. ANS increases its quantum yield in response to aggregate size, which is rather surprising given that more accessible surface area is provided in monomeric molten-globule states
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. Whenever possible, the number of vertebrae and numbers of dorsal, anal, caudal, pectoral, and pelvic fin rays were recorded
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. [1] were (1) nucleation rates for LLPS and crystallization, (2) relative position of LLPS and crystallization temperature, (3) time provided by the thermal cycle for nucleation of phases during LLPS before nucleation of crystallization occurs, (4) relative positions of spinodal and binodal regions (nucleation rate of LLPS phases), and (5) growth of phases after nucleation (especially LLPS [1, 10, 15])
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. This study suggests that the use of a defined medium containing TGF-[beta] is necessary to induce the re-expression of a differentiated chondrocytic phenotype and the subsequent stimulation of glycosaminoglycan and type-II collagen production by human monolayer expanded chondrocytes
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. Segments or regions that are directly involved in intermolecular interactions or that are indirectly affected upon binding: residue F43 of CD4 (code: F43) and the residues of gp120 forming a cavity to hold F43 (code: F43cav), residues of gp120 that form intermolecular hydrogen bonds (code: interHb) and their counterparts in CD4 (code: interHb); see Fig
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. This feature of FRET has been used extensively to study the structure and dynamics of biological molecules and assemblies, in ensemble as well as more recent single-molecule measurements (Selvin 2000; Klostermeier and Millar, 2001b; Weiss, 2000; Ha, 2001)
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