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. Of particular interest is the value of R^sub o^, which is related to the persistence length and also provides a measure of the goodness of the solvent because R^sup 3^^sub o^ quantifies the average volume per residue set aside by the chain for interactions with the surrounding solvent (38,41)
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. Dimensions of denatured protein chains from hydrodynamic data
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. In EI^sup sc^ at low pH, the fluorescence intensity of ANS was largely enhanced, and the maximum wavelength of the fluorescence spectrum was 480 nm (Fig
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. Plenum Press, New York
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. Curr Biol 2004; 14:2208-2216(34) Fadok VA, Bratton DL, Guthrie L, et al
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. Chest 2004; 125:70S-78S(62) Decralner M, Rutten-van Molken M, Dekhuijzen PN, et al
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. METHODSSubjects and Data CollectionThe subjects were students enrolled in fall semester 2001 courses across the first three years of the Pharm
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. The fluorescence emission of native GR is quite blue-shifted ([lambda]^sub max^ at 317 nm), indicating that, on average, the four tryptophan residues in each GR subunit are well-protected from exposure to the aqueous medium in the folded protein
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. The average shape of a denatured protein is that of a prolate ellipsoid We computed the ensemble-averaged asphericity values for each of the 23 sequences in the EV limit and the resultant data are shown as cross marks in Fig
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. CONCLUSIONS We have used an atomistic EV model, developed in previous work, to show that it is computationally tractable to generate accurate conformational ensembles for proteins in the EV limit
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