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. The probability of linding a pair of residues in close spatial proximity decreases with increasing sequence separation
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. For a Flory random coil, P(x) is a Gaussian distribution of the form P(x) = (3/2π)^sup 3/2^exp(-1
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. Once the first scan was completed, the samples were cooled down in situ to 283 K for 15 min and rescanned under the same experimental conditions to test for the reversibility of the denaturation reaction
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. Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2
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. The stabilization of state C is probably due to an increase of its entropy, related to larger MSDs (in line with neutron scattering experiments on denatured α-amylase, (51))
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. 07, and are fairly uniform throughout the sequence, except for five outliers with S^sup 2^^sub s^ values between 0
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. Its characteristic features are: i), specific high-affinity binding in the presence of fast exchange, ii), accommodation of a relatively nonmatching and flexible target via exchange between multiple binding alternatives, iii) dimerization of CaM upon target binding, and iv), large-scale (domain) motions on the nanosecond and microsecond timescale
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. Basic helix-loop-helix protein sequences determining differential inhibition by calmodulin and S-100 proteins
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. 6 kDa and a protein specific volume of 0
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. 4) are plotted as a function of residue number in Fig
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. These data were used to estimate the location of the binodal curve so that we could better understand the effect of dissolving temperature on particle size
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. The cleavage reaction was initiated by addition of substrate strands (500 pM) to the respective preformed ribozyme complex (100 nM) at room temperature and reaction progress was monitored by gel analysis of aliquots quenched with formamide at different time points
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. ProcedurePermission from the human subjects review board was granted to conduct the current study
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. The fluorescence emission of GR undergoes a marked red-shift between 1 and 3 M GdnHCl (with a transition midpoint at ~2
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