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. In brief, all FTIR experiments were performed on a Perkin-Elmer Spectrum 2000 spectrometer (Wellesley, MA) equipped with a dTGS detector
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. Formally, folding can be viewed as a symmetry-breaking operation wherein the dilatation symmetry characteristic of the denatured-state (EV limit) ensemble is broken by breaking or disrupting the requisite number of nonnative local contacts
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. and acquired at pH 3
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. In steady shear, the zero shear viscosity of concentrated solutions at pH 5
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. We compared the properties of DCVJ with the known amyloid specific dye ThT and found striking similarities, including fluorescence lifetimes and viscosity dependence on fluorescence intensity
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. The Sf9 expression system allows for postsynthetic modifications of the proteins
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. This suggests that the cooperativity unit in this transition comprises a single copy of lipidated apoA-2 rather than the entire lipoprotein
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. A lookup table was developed from this experiment and included with the part program
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. At low Mg^sup 2+^, all three ribozymes show a single peak at low E, reflecting an extended, undocked state
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. Anne Holbrook is the recipient of a CIHR Career Investigator Award; Adrian Levy is supported by a Scholar award from the BC Michael Smith Foundation for Health Research
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. Fully unfolded monomers were obtained at higher concentrations of GdnHCl
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. Experiments that only detect close spatial contacts will be interpreted as providing evidence of long-range "residual" structure in the denatured state (94-98)
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. A Gaussian-chain model for treating residual charge-charge interactions in the unfolded state of proteins
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.1°C/min) gives rise to less pronounced aggregation during the thermal unfolding transition as compared to higher heating rates; and (2), the lowest protein concentration (7 µg/ml) reveals a less-pronounced aggregation also for a higher heating rate (1°C/min), whereas higher protein concentrations studied with the higher heating rate exhibit more pronounced aggregation
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.Abbreviations used: PLC, mammalian phosphoinositide-specific phospholipase C; PI(4,5)P^sub 2^, phosphatidylinositol 4,5- bisphosphate; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; POPS, 1-palmitoyl-2-oleoylphosphatidylserine; PE, 1-palmitoyl-2-oleoylphosphatidylethanolamine; PH, pleckstrin homology; DNS-PE, dansyl phosphadtiylethanolamine; FRET, fluorescence resonance energy transfer
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.5 h at 75°C) or at higher temperatures (30 min at 95°C) leads to an increased population of large multilamellar liposomes (Fig
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