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. Inactivation-reactivation of two-electron reduced Escherichia coli glutathione reductase involving a dimer-monomer equilibrium
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. Infrared spectra of amide groups in α-helical proteins: evidence for hydrogen bonding between helices and water
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. The stability of these mutants is ~3 kcal/mole lower relatively to the WT (20-22), and the internal flexibility of the polypeptide is considerably larger, particularly with I7S and F110S (18)
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. Ice-induced ANS binding to other proteins To examine whether the ice-induced perturbation observed for azurin is common to proteins in general, ANS binding experiments were extended to a set of seven other proteins including monomeric BSA, α-amylase and β-gal, dimeric LADH and tetrameric ALD, YADH, and LDH
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. For magic angle spinning (MAS) experiments, an indirect detection pulsed field gradient nano H{X} probe (4 mm) was used in a Varian (Palo Alto, CA) UNITY INOVA 500 NMR Spectrometer
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. Backbone dynamics of calmodulin studied by N-15 relaxation using inverse detected 2-dimensional NMR-spectroscopy: the central helix is flexible
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. It should however be noted that Bis-ANS still fluoresces very strongly in the presence of large aggregates (Figs
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. CONCLUDING REMARKSGiven the severity of many diseases associated with protein misfolding and aggregation, including the amyloidoses and the transmissible spongiform encephalopathies, research in this area is intense
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. Also, since apolipoprotein ability to prevent HDL fusion with the cell membrane is proposed to play a key role in the receptor-mediated selective cholesterol uptake from HDL (Thuahnai et al
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. 7 nm), and their surface appears to be structured with 6-8 subcords aligned in parallel, which are likely to be protofilaments of an individual height of ~2
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. Although considerable evidence suggests that only partial destabilization of insulin is the prerequisite for its aggregation (Ahmad et al
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. Manuscript received July 8, 2003; accepted October 24, 2003
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