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Keep on the Sunny Side collects 40 June Carter Cash performances dating from 1939 to 2003, from Carter Family through collaborations June did with Homer and Jethro, Cal Smith and, of course, Johnny Cash, plus solo work
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. The average shape of a denatured protein is that of a prolate ellipsoid We computed the ensemble-averaged asphericity values for each of the 23 sequences in the EV limit and the resultant data are shown as cross marks in Fig
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. Reversible equilibrium unfolding of triosephosphate isomerase from Trypanosoma cruzi in guanidinium hydrochloride involves stable dimeric and monomeric intermediates
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. Physicochemical background for the fluorescence properties of DCVJ and ThTTo understand the physicochemical background for the fluorescence of DCVJ and ThT when bound to misfolded TTR aggregates, we used glycerol-water mixtures to decrease the intrinsic rotational relaxation time of the fluorescent dyes
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. 5-bisphosphate and the α and βγ subunits of G-proteins
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. The Colorocs toner has melted and coated individual fibers; no toner film is visible in the SEM, while the solvent dye is clearly present on the fibers, as seen in the OM picture (reproduced here in black and white; the dye is a red color and can be clearly seen in the color originals)
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. [FIGURE 3, 4 & 5 OMITTED]FecundityAbsolute fecundity for prefertilization female black rockfish ranged from 482,528 oocytcs for a 5-year-old female to 998,050 oocytes for a 19-ycar-old female
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. About 50 mL of antibody solution was obtained after two weeks of cultivation
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- All this might be less disturbing had Carter confined his post-presidential efforts to such good works as vanquishing the guinea worm
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. Ligand-protein contacts (LPC) was derived with LPC software: automated analysis of interatomic contacts in proteins
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. Accurate models for denatured states also impact a range of areas, including quantitative studies of in vitro folding pathways (10,19-24), protein design (25,26), studies of protein aggregation (27-29), and understanding preferential interactions in cosolute mixtures (1,3,30-36)
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. Of course, what we refer to as cavities will actually be filled by solvent and cosolule molecules under denaturing conditions
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.A schematic representation of the TTR misfolding reaction from the unfolded state through the A-state is presented in Fig
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. Bound to aggregated TTR, the fluorescence lifetime of both components have increased and the relative amplitude of the slower component has increased (Table 3), well in accordance with the massive increase in quantum yield compared to when bound to native TTR (Fig
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. In contrast, the specifically-docked conformation (exemplified by the WT 4WJ) is stabilized by an extensive hydrogen bond network (14 hydrogen bonds in all (Rupert and Ferre-D'Amare, 2001)), prolonging the lifetime of this state
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