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5 M, the GR dimer eluted at a volume corresponding to ~120 kDa
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It was suggested, with the help of some computational studies, that this enhanced stability of the tetramer is mainly due to glycosylation and some stabilizing ionic interactions at the oligomeric interface (18)
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The latter refers to conditions under which the conformational ensemble can be modeled by ignoring all interactions except "two-body" repulsive (steric) interactions of the excluded volume (EV) kind
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1 is used) assuming the apparent specific volume of the protein phase to be 1
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Theoretically the occurrence of particle gel formation is linked with percolation although this is a necessary rather than a sufficient condition for gelation to occur (21)
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As we discussed previously (13), the behavior in this study is relevant to globular proteins subjected to osmotic stress in biological situations
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Despite limited accuracy in the particle size determination and limited resolution, this system facilitates observation of lipoproteins, lipid-free and lipid-poor proteins on the same gel and is widely used for lipoprotein analysis (Davidson et al
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Moreover, deposition of the tiny aqueous droplets on the mica surface is followed by very fast (timescale of seconds) evaporation of bulk solvent, which eventually prevents further aggregation
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T^sub g^ and initial modulus were determined by dynamic mechanical analysis (DMA, Seiko SII DMS 210)
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