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Circular dichroismFar-UV CD spectra of GR were measured on a JASCO (Tokyo, Japan) J-715 spectropolarimeter (kindly made available by Dr
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In this research, the sequential events in ACYI thermal unfolding were investigated by a combination of spectroscopic methods and the related resolution-enhancing techniques
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Interestingly, the difference in T^sub m^ of the two forms is ~26°C, and this estimates the extent of stability imparted by the oligosaccharide chains to the stability of gSBA
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Amyloid fibrils from the viewpoint of protein folding
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fluorescent residues are involved in the self-association interface: and, 3), the value of 1 µM could be considered an upper estimation of the EI^sup sc^ self-dissociation constant
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It is tempting to suggest, based on our results, that the species populated at high pH is also a molten-globule-like species, although it did not bind ANS (Fig
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The Saccharomyces cerevisiae nucleoporin Nup2p is a natively unfolded protein
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However, it has been shown in various cases that (and how) under specific conditions λ^sub max^-signals can be used for quantitative analyses of unfolding transitions (4,12,15)
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Comparison of solute-induced protein stabilization in aqueous solution and in the frozen and dried states
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We find that the K^sub d^ value for this interaction in solution is higher than expected based on a simple model where we calculate protein association on the reduced volume of the membrane
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Only the fast phase is observed upon repetitive heating, suggesting that lipid-poor protein and protein-containing vesicles have lower kinetic stability than the disks
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The thermodynamics of the self-association of the reduced and carboxymethylated form of apoA-II from the human high density lipoprotein complex
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If it is too high, the parts are quarantined in a queue until they are cool enough for machining
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These phase separations generate different morphologies caused by the phase stability and diffusion during the separation
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The low molecular weight IPP polymers produced smaller particles than the high molecular weight polymers
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If an order is submitted by automated voice response or online, a Medco pharmacist will contact the client's doctor and confirm the patient is eligible for the medication
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. Besides, the lack of lipid signal in the nucleus is consistent with the lack of yolk platelets in the nucleus (31,32) and with a previous lipid ^sup 1^H MRI study of the Xenopus oocyte (12)
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.In conclusion, we have shown that insulin amyloidogenesis in vitro induced by the en masse destabilization of native dimers proceeds through a multipathway assembling scheme, which employs-on the one hand-the hierarchical intertwisting reported earlier; and on the other hand, employs lateral interactions of single particles and fibrils, leading to larger domains of features aligned in parallel (Fig
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.The "diffusion-collision" approach, on the other hand, is supported by strong experimental evidence that folding rates are controlled by the rate of diffusion of pieces of the open coil in their search for favorable contacts, rather than a driven collapse along some continuous energy surface (Jacob et al
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