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. 1°C/min reveals for all protein concentrations λ^sub max^-values around 345 nm for the unfolded state
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. This has been exploited in diffusion-weighted MRS to differentiate between intracellular and extracellular signal contributions of nonwater molecules (17,18)
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. Bis-ANS shows a wide variety of fluorescence responses and fluoresces most strongly when bound to monomeric A-state TTR and surprisingly also fluoresces strongly when bound to unfolded monomeric TTR
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. Once protein unfolding reached completion in the first jump, the sample was cooled and incubated at 25°C for 1000 s, which was sufficient for completion of the helical refolding (as indicated by flattening of the Θ^sub 222^(t)) but not for the disk reconstitution (as indicated by DSC, EM, and gel electrophoresis)
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