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. This suggested that the two domains of ACYI might fold hierarchically and the folding of the catalytic domain is the rate-limiting step
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. The spectra recorded at temperatures from 68°C to 72°C were not included in the 2D IR analysis in Fig
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. Monoclonal antibody based immunoassay for human aminoacylase-1
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. The solvent-accessibility of tryptophan and tyrosine residues was examined by iodide and acrylamide quenching at several pHs
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. This exemplifies that the correct criterion for thermodynamic reversibility is the absence of scan rate effects and hysteresis rather than the repeatability of a transition
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. Control samples (freshly cleaved mica, mica, and aqueous HCl-solution) were also investigated with AFM to exclude possible artifacts
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